The use of Artemia nauplii for toxicity test: a critical analysis
Sorgeloos, P.; Remiche-Van der Wielen, C.; Persoone, G. (1978). The use of Artemia nauplii for toxicity test: a critical analysis. Ecotoxicol. Environ. Saf. 2: 249-255
In: Ecotoxicology and Environmental Safety. Academic Press/Elsevier: Amsterdam, Netherlands etc. ISSN 0147-6513; e-ISSN 1090-2414, more
Because of their easy hatching from dry cysts and their year-round availability, nauplii of the brine shrimp Artemia salina are most convenient test organisms for toxicity studies. The bioassays mentioned in literature have, however, mostly been carried out with larvae of which neither the exact instar stage nor the geographical origin is known. The incubation of the cysts should always be carried out under strictly controlled temperature conditions since both the onset of hatching and the length of the hatching period are temperature-dependent. Moreover, the experiments should only be carried out with populations of nauplii of the same stage of development since it is demonstrated that the second and third instars are significantly more sensitive to toxicants than first instar larvae. It is demonstrated that the sensitivity to chemicals varies from one geographical Artemia strain to another.
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