Peroxide-triggered erythrocytes haemolysis as a model for the study of oxidative damage in marine fishes
Janssens, B.J.; Le Gall, R.; Rees, J.F. (2002). Peroxide-triggered erythrocytes haemolysis as a model for the study of oxidative damage in marine fishes. J. Fish Biol. 61(1): 71-84. dx.doi.org/10.1006/jfbi.2002.2008
In: Journal of Fish Biology. Fisheries Society of the British Isles: London,New York,. ISSN 0022-1112; e-ISSN 1095-8649, meer
The haemolysis of sea bass Dicentrarchus labrax red blood cells (RBC) was initiated by tert-butyl-hydroperoxide (t-BHP). The onset of the haemolytic process, as accelerated by increasing t-BHP concentration, This process as preceded by a drop in the RBC glutathione content followed by the production of lipid peroxidation products. Also t-BHP induced DNA fragmentation in RBC nuclei as measured by COMET assay. The addition of the antioxidant Trolox C-R dose-dependent delayed the onset of both lipid peroxidation and haemolysis, and protected GSH stores against t-BHP-induced depletion. DNA fragmentation was also prevented by Trolox C-R. These results indicate that L-BHP induces haemolysis in sea bass RBC through the induction of oxidative stress Such a simple model Could prove useful for both fundamental and applied studies on marine fish antioxidant mechanisms.
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