Immunocytochemical identification and localization of the different cell-types in the pituitary of the seabass (Dicentrarchus labrax)
Cambre, M.L.; Verdonck, W.; Ollevier, F.; Vandesande, F.; Batten, T.F.C.; Kuhn, E.R. (1986). Immunocytochemical identification and localization of the different cell-types in the pituitary of the seabass (Dicentrarchus labrax). Gen. Comp. Endocrinol. 61(3): 368-375. https://dx.doi.org/10.1016/0016-6480(86)90222-4
In: General and Comparative Endocrinology. Elsevier: New York,. ISSN 0016-6480; e-ISSN 1095-6840, meer
Antisera raised against chum salmon prolactin (PRL), trout growth hormone (GH), mammalian adrenocorticotropic hormone (ACTH), thyroid-stimulating hormone (TSH), luteinizing hormone (LH), and α-melanophore-stimulating hormone (α-MSH) were used to localize PRL, GH, ACTH, gonadotropic, TSH, and MSH cells in the hypophysis of the teleost Dicentrarchus labrax using the unlabeled peroxidase anti-peroxidase method. In the rostral pars distalis, ACTH cells stained very intensively with anti-ACTH; so did the MSH cells in the pars intermedia. The prolactin cells stained very specifically with anti-prolactin without staining the growth hormone cells. In the proximal pars distalis anti-GH, anti-TSH β, and anti-LH stained selectively the corresponding cells; with these antisera no cross-reaction with any other cell type was observed. Anti-α-MSH only stained cells in the pars intermedia. Some cells in the pars intermedia did not react at all; these could correspond to the PAS-positive cells. A characteristic feature was positive staining with anti-LH in some cell groups encircling the pars intermedia, indicating the fact that in the seabass some cells of the proximal pars distalis surround the pars intermedia.
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