Surveys for bacteriological analysis were carried out in 1990-1991 in two different marine fish hatcheries located in Greece and northern Spain. Samples were taken from 12 different routine productions of Brachionus plicatilis before and during enrichment. Isolates were characterized by whole cell fatty acid methyl ester analysis and by BIOLOG metabolic fingerprinting. Vibrio anguillarum and/or Vibrio alginolyticus were dominant Vibrio species in the rotifer samples taken after enrichment and rinsing, before being delivered to the fish larvae. None of the Vibrio anguillarum strains belonged to the pathogenic serotypes for juvenile and adult fish.
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