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Genetic study Ameiurus sp. in Flanders and neighbouring regions

Archived data
Availability: Unrestricted after moratorium period
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Description
The dataset contains genetic data from Ameiurus specimens collected in Flanders, Wallonia, France and the Netherlands. more

The dataset contains data from Ameiurus specimens collected in Flanders, Wallonia, France and the Netherlands. Fin clips from these specimens were prepared for genetic analysis (DNA amplicfication followed by sequence reaction). To clear the uncertainty about the identification of A. melas and A. nebulosus in Flanders we sampled Ameiurus fin clips from eleven sites in Flanders, and for comparison one in Wallonia, three in The Netherlands and one in France for genetic study. A 650 basepair fragment of the 5’ end of the mitochondrial Cytochrome Oxidase I (COI) gene (bar-coding region) was analysed using universal COI primer cocktails with M13 tails. For species identification, the obtained sequences were compared to published sequences in Genbank.

Scope
Themes:
Biology > Fish
Keywords:
Fresh water, Ameiurus melas, Ameiurus nebulosus, Genetic screening, Species identification, Belgium: Flanders, France, Netherlands, Belgium, Europe, Ameiurus melas (Rafinesque, 1820), Ameiurus nebulosus (Lesueur, 1819)

Geographical coverage
Belgium: Flanders
France Stations [Marine Regions]
north part
Netherlands Stations [Marine Regions]
south part
Belgium [Marine Regions]

Temporal coverage
23 June 2008 - 15 June 2010

Taxonomic coverage
Ameiurus melas (Rafinesque, 1820) [WoRMS]
Ameiurus nebulosus (Lesueur, 1819) [WoRMS]

Parameters
Species identification Methodology
Total length
Weight
Species identification: Genetic analysis

Contributors
Vlaamse overheid; Beleidsdomein Leefmilieu, Natuur en Energie; Instituut voor Natuur- en Bosonderzoek; Afdeling Biodiversiteit en Natuurlijk Milieu; Onderzoeksgroep Soortendiversiteit, moredata creator
Vlaamse overheid; Beleidsdomein Omgeving; Instituut voor Natuur- en Bosonderzoek (INBO), moresample analyserdata owner

Related datasets
Other relation:
Morphological study of Ameirus sp. in Flanders, more

Project
Genetic and morphological study of two Ameirus species in Flanders and neighbouring regions, more

Dataset status: Completed
Data type: Data
Data origin: Research: experiment
Metadatarecord created: 2010-10-26
Information last updated: 2010-10-26
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