IMIS - Marine Research Groups | ||||
Pelagic microbes (Eukaryote 18S and Material 16S amplicons) from sites in the Southern, Indian and Arctic Oceans
Citation
Schroeder D, Lebret K, Balestreri C, Highfield A, Schroeder J, Thorpe S, Moore K, Pasckiewicz K, Pfaff M, Rybicki E, Flaviani F (2018): Pelagic microbes (Eukaryote 18S and Material 16S amplicons) from sites in the Southern, Indian and Arctic Oceans. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=pelagic_microbes_chlorophyll_maximum_southern_indian_arctic_oceans&v=1.2 https://doi.org/10.15468/x2spov
Contact:
Flaviani, Flavia Availability:
![]() Description
Amplicon sequencing dataset targeting Eukaryotes (18S V9 region) and Bacteria (16S V4 region) in the <0.45μm fraction of pelagic microbes, sampled in the chlorophyll maximum zone. more
At each station, 1 l of seawater from the chlorophyll maximum layer was sampled by a conductivity temperature depth (CTD) rosette sampler on-board the R/V Roger Revelle. An aliquot of 250 ml was filtered through a 0.45-μm polycarbonate filter. DNA extraction of material retained on the filter was performed using the Qiagen DNeasy Blood and Tissue protocol (QIAGEN, Valencia, CA, United States). The DNA was stored at −21°C and subsequently transferred to Plymouth, United Kingdom, for further processing. An additional 50 ml sample of filtered water was stored at 4°C in the dark for further processing in the laboratory after the cruise. Point measurement samples were collected during the Great Southern Coccolithophore Belt expedition (GSCB-cruise RR1202). Stations S1 and S2 were located in the South-West Indian Ocean, stations S3 and S4 in the Southern Ocean, and stations S5 and S6 in the South-East Indian Ocean. Method step description:
Scope Themes: Biology > Plankton Keywords: Marine/Coastal, Eukaryotes, Metadata, Pelagic zone, ISW, Indian Ocean, PS, Antarctic Ocean, PS, Southern Ocean, Bacteria Geographical coverage ISW, Indian Ocean [Marine Regions] PS, Antarctic Ocean [Marine Regions] PS, Southern Ocean [Marine Regions] Temporal coverage
6 March 2012 - 14 April 2013 Taxonomic coverage
Bacteria [WoRMS]
Parameter
Molecular data Contributors
University of Cape Town, more, data creator
Marine Biological Association of the UK (MBA), more, data creator
Natural Environment Research Council; British Antarctic Survey (BAS), more, data creator
University of Exeter; Exeter Sequencing Service, more, data creator
Department of Environmental Affairs (DEA), more, data creator
Related datasets
Dataset status: Completed
Data type: Metadata
Data origin: Research: field survey
Metadatarecord created: 2018-12-12
Information last updated: 2019-04-10
|