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Immunostimulatory effects of marine bacteria and bacterial endotoxin in sea spray aerosols
Citation
Schütte, W., Li, Y., De Rijcke, M. (2023): Immunostimulatory effects of marine bacteria and bacterial endotoxin in sea spray aerosols. Flanders Marine Institute (VLIZ). https://marineinfo.org/id/dataset/8344
Contact: De Rijcke, Maarten ;
Archived data
Availability: The data can be made available upon request, please use contact information.
Description

Bacterial counts and endotoxin concentrations of aerosol samples collected at the beach of Ostend, Belgium, and generated in the laboratory with a marine aerosol referenc tank (MART). Data regarding gene induction in human reporter cell lines (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6, THP1-Dual) after stimulation with these collected aerosols.

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The data was used to examine levels of bacterial endotoxins in SSA and their effect on cell-signalling pathways of the human innate immune system. Human reporter cell lines (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6 and THP1-Dual) were exposed to aerosol samples collected at the beach of Ostend (Belgium) and generated with a Marine Aerosol Reference Tank (MART) in the laboratory. Bacteria cell count s in aerosol samples were measured with flow cytometry and endotoxins were quantified using the rFC assay.

Scope
Keywords:
Immunology, Marine ecology, Sea spray, Sea spray aerosols, ANE, Belgium, Oostende
Geographical coverage
ANE, Belgium, Oostende [Marine Regions]
Temporal coverage
3 March 2023 - 26 May 2023
Parameters
Count of bacteria Methodology
Endotocin concentration Methodology
Gene induction measured at optical density 405nm Methodology
Count of bacteria: Flow cytometry

Endotocin concentration: rF C assay
rF C assay
Gene induction measured at optical density 405nm: Reporter cell lines assay (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6, THP1-Dual cells)
Reporter cell lines assay (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6, THP1-Dual cells)
Contributors
Universiteit Gent; Faculteit Wetenschappen, more, data creator
Schütte, Wyona
Vlaams Instituut voor de Zee (VLIZ), more, data creator, data creator
Li, Yunmeng
De Rijcke, Maarten
Dataset status: Completed
Data type: Data
Data origin: Research: experiment
Metadatarecord created: 2023-06-02
Information last updated: 2023-06-02
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy